LOCUS       sequence-149047-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of NEK1. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: NEK1_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggagaagt atgttagact acagaagatt ggagaaggtt catttggaaa agccattctt
       61 gttaaatcta cagaagatgg cagacagtat gttatcaagg aaattaacat ctcaagaatg
      121 tccagtaaag aaagagaaga atcaaggaga gaagttgcag tattggcaaa catgaagcat
      181 ccaaatattg tccagtatag agaatcattt gaagaaaatg gctctctcta catagtaatg
      241 gattactgtg agggagggga tctgtttaag cgaataaatg ctcagaaagg cgttttgttt
      301 caagaggatc agattttgga ctggtttgta cagatatgtt tggccctgaa acatgtacat
      361 gatagaaaaa ttcttcatcg agacattaaa tctcagaaca tatttttaac taaagatgga
      421 acagtacaac ttggagattt tggaattgct agagttctta atagtactgt agagctggct
      481 cgagcttgca tagggacccc atactacttg tcacctgaaa tctgtgaaaa caaaccttac
      541 aataataaaa gtgacatttg ggctctgggg tgtgtccttt atgagctgtg tacacttaaa
      601 catgcttttg aagctggcag tatgaaaaac ctggtactga agataatatc tggatctttt
      661 ccacctgtgt ctttgcatta ttcctatgat ctccgcagtt tggtgtctca gttatttaaa
      721 agaaatccta gggatagacc atcagtcaac tccatattgg agaaaggttt tatagccaaa
      781 cgcattgaaa agtttctctc tcctcagctt attgcagaag aattttgtct aaaaacattt
      841 tcgaagtttg gatcacagcc tataccagct aaaagaccag cttcaggaca aaactcgatt
      901 tctgttatgc ctgctcagaa aattacaaag cctgccgcta aatatggaat acctttagca
      961 tataagaaat atggagataa aaaa
//
