LOCUS       sequence-149072-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  One of four plasmids needed to create the quadruple Holliday 
            junction substrate (QHJS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Chen SH, Plank JL, Willcox S, Griffith JD, Hsieh TS
  TITLE     Improved methods for creating migratable Holliday junction 
            substrates.
  JOURNAL   Nucleic Acids Res. 2013 Mar 1;41(5):e60. doi: 10.1093/nar/gks1343. 
            Epub 2012 Dec 28.
  PUBMED    23275569
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2013-03-1"; volume: "41(5):e60. doi"; pages: " 
            10.1093/nar/gks1343. Epub 2012 Dec 28"
COMMENT     Sequence Label: pDHJS4 AS+
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     186..208
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     200..217
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     201..217
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     224..243
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        224..242
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     267..283
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     268..284
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    308..341
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        complement(850..868)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(889..905)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(889..905)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(902..924)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    913..929
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(937..943,944..961,962..967))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
       61 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
      121 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      181 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
      241 gggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgataagc tagatctcgc
      301 ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactgaatt caggaccttg
      361 aacgcgaggt gcgcggctgt caggattacg cgtgattatc tggcccgggc tactcggtgt
      421 acaacaaggt cttcgatttc gaggcccctg tgcgcggtca gaatgccaaa atggttatga
      481 cctccgtatc cggccacatg atgcagctgg cattccaggt gtcttacaaa aattggagaa
      541 cggtggatcc gcgctcttta ttcgacgcgc cggtggaaaa gggcgtgggc tcggattatg
      601 agcctatcaa aaggaccttg gaacgcgagg tgcgcggctg tcagggattg attatctgga
      661 cggattgcga tcgcgagggc gagaacattg gctacgagat tattgatgtg cggccgcatc
      721 cgagccattg gaagattagg ttctgcatgc gcacacaatt gatgacttga tctagagtag
      781 tgccccaact ggggtaacct ttgagttctc tcagttgggg gcgtaggata tgagctccag
      841 cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt
      901 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
      961 gtgtaaagcc tggggtgcct aatgagtgag ctaa
//
