LOCUS       sequence-149074-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  One of four plasmids needed to create the quadruple Holliday 
            junction substrate (QHJS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Chen SH, Plank JL, Willcox S, Griffith JD, Hsieh TS
  TITLE     Improved methods for creating migratable Holliday junction 
            substrates.
  JOURNAL   Nucleic Acids Res. 2013 Mar 1;41(5):e60. doi: 10.1093/nar/gks1343. 
            Epub 2012 Dec 28.
  PUBMED    23275569
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2013-03-1"; volume: "41(5):e60. doi"; pages: " 
            10.1093/nar/gks1343. Epub 2012 Dec 28"
COMMENT     Sequence Label: pDHJS4 AS+
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    88..109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(124..129,130..147,148..154)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    162..178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     167..189
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     186..202
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     186..202
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        223..241
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    750..783
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     primer_bind     complement(807..823)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(808..824)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(848..867)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(849..867)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(874..891)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(874..890)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(883..905)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
       61 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
      121 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
      181 tcacacagga aacagctatg accatgatta cgccaagctc gaaattaacc ctcactaaag
      241 ggaacaaaag ctggagctca tatcctacgc ccccaactga gagaactcaa aggttacccc
      301 agttggggca ctactctaga tcaagtcatc aattgtgtgc gcatgcagaa cctaatcttc
      361 caatggctcg gatgcggccg cacatcaata atctcgtagc caatgttctc gccctcgcga
      421 tcgcaatccg tccagataat caatccctga cagccgcgca cctcgcgttc caaggtcctt
      481 ttgataggct cataatccga gcccacgccc ttttccaccg gcgcgtcgaa taaagagcgc
      541 ggatccaccg ttctccaatt tttgtaagac acctggaatg ccagctgcat catgtggccg
      601 gatacggagg tcataaccat tttggcattc tgaccgcgca caggggcctc gaaatcgaag
      661 accttgttgt acaccgagta gcccgggcca gataatcacg cgtaatcctg acagccgcgc
      721 acctcgcgtt caaggtcctg aattcagtac gcgcccgggg agcccaaggg cacgccctgg
      781 cacccgcacc gcgagatcta gcttatcgat accgtcgacc tcgagggggg gcccggtacc
      841 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga
      901 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
      961 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     1021 tggcgaatgg a
//
