LOCUS       sequence-149075-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  One of four plasmids needed to create the mismatched double Holliday
            junction substrate (MM-DHJS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Plank JL, Hsieh TS
  TITLE     A novel, topologically constrained DNA molecule containing a double 
            Holliday junction: design, synthesis, and initial biochemical 
            characterization.
  JOURNAL   J Biol Chem. 2006 Jun 23;281(25):17510-6. Epub 2006 Apr 11.
  PUBMED    16608853
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2006-06-23"; volume: "281(25)"; pages: "17510-6. Epub
            2006 Apr 11"
COMMENT     Sequence Label: pDHJS3 AN+
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    89..110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(125..130,131..148,149..155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    163..179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     168..190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     187..203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     187..203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        224..242
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    266..299
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    731..764
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(778..794)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(779..795)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(819..838)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(820..838)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(845..862)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(845..861)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(854..876)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
       61 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      121 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      181 ttcacacagg aaacagctat gaccatgatt acgccaagct cgaaattaac cctcactaaa
      241 gggaacaaaa gctggagctc atatgataac ttcgtataat gtatgctata cgaagttatt
      301 ctagatcaag tcatcaattg tgtgcgctag cagaacctaa tcttccaatg gctcggatgc
      361 ggccgcacat caataatctc gtagccaatg ttctcgccct cgcgatcgca atccgtccag
      421 ataatcaatc cctgacagcc gcgcacctcg cgttccaagg tccttttgat aggctcataa
      481 tccgagccca cgcccttttc caccggcgcg tcgaataaag agcgcggatc caccgttctc
      541 caatttttgt aagacacctg gaatgccagc tgcatcatgt ggccggatac ggaggtcata
      601 accattttgg cattctgacc gcgcacaggg gcctcgaaat cgaagacctt gttgtacacc
      661 gagtagcccg ggccagataa tcacgcgtaa tcctgacagc cgcgcacctc gcgttcaagg
      721 tcctgaattc ataacttcgt ataatgtatg ctatacgaag ttatagatct agcttatcga
      781 taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag tcgtattaca
      841 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      901 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      961 atcgcccttc ccaacagttg cgcagcctga atggcgaatg g
//
