LOCUS       sequence-149078-         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Burger A, Lindsay H, Felker A, Hess C, Anders C, Chiavacci E, Zaugg 
            J, Weber LM, Catena R, Jinek M, Robinson MD, Mosimann C
  TITLE     Maximizing mutagenesis with solubilized CRISPR-Cas9 
            ribonucleoprotein complexes.
  JOURNAL   Development. 2016 Apr 29. pii: dev.134809.
  PUBMED    27130213
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2016 Apr 29. pii: dev.134809."
COMMENT     Sequence Label: pMJ922
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..70)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(77..97)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(110..136)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(116..142)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccttgctcac ggatccgctg ctgccgttgc tcccactacc aatgccgtcc actttccgct
       61 ttttcttagg atcttccacc ttgcgttttt tcttggggga gccgctgccc aggctggcat
      121 aatcgggcac gtcataggga taggcgtcgc ctcccagctg agacaggtcg atccgtgtct
      181 cgtacaggcc ggtgatgctc tggtggatca gggtggcgtc cagcacctct ttggtgctgg
      241 tgtacctctt ccggtcgatg gtggtgtcaa agtacttgaa ggcggcaggg gctcccagat
      301 tggtcagggt aaacaggtgg atgatattct cggcctgctc tctgataggc ttgtctctgt
      361 gcttgttgta ggcgctcagc accttgtcca gattagcgtc ggccaggatc actctcttgg
      421 agaactcgct gatctgctcg atgatctcgt ccaggtagtg tttgtgctgt tccacaaaca
      481 gctgtttctg ctcattatcc tcgggggagc ccttcagctt ctcatagtgg ctggccaggt
      541 acaggaagtt cacatatttg gagggcaggg ccagttcgtt tcccttctgc agttcgccgg
      601 cagaggccag cattctcttc cggccgtttt ccagctcgaa cagggagtac ttaggcagct
      661 tgatgatcag gtcctttttc acttctttgt agcc
//
