LOCUS       sequence-149079-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Burger A, Lindsay H, Felker A, Hess C, Anders C, Chiavacci E, Zaugg 
            J, Weber LM, Catena R, Jinek M, Robinson MD, Mosimann C
  TITLE     Maximizing mutagenesis with solubilized CRISPR-Cas9 
            ribonucleoprotein complexes.
  JOURNAL   Development. 2016 Apr 29. pii: dev.134809.
  PUBMED    27130213
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2016 Apr 29. pii: dev.134809."
COMMENT     Sequence Label: pMJ922
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..50
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      54..63
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ataacaataa caacaacctc gggatcgagg aaaacctgta cttccaatcc aatgccacca
       61 tggacaagaa gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga
      121 tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc
      181 acagcatcaa gaagaacctg atcggcgccc tgctgttcga cagcggagaa acagccgagg
      241 ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct
      301 atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac
      361 tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca
      421 acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga
      481 aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca
      541 tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg
      601 tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca
      661 tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagac
      721 ggctggaaaa tctgatcgcc
//
