LOCUS       sequence-149083-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Creating LexA::GADfluw lines from MiMIC lines with inserts into 
            coding introns in Phase 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..29
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11..29
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    69..138
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 gccagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccatg catgatgtag
       61 gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg
      121 tactccacct cacccatctg gtccatcatg atggtcgaca agcttagaag ttcaaatggg
      181 ctttcatatt tacttttttc tctgagtggg taaggtcatt gtggggtctt aagggctaaa
      241 gggtagtgat gatgaaggca tttgatgcca ttgttcaata acttacctgc cggaacctcc
      301 gcttccaccg ctacctccgg atctttaatt aaaaacgagt ttttaagcaa actcactccc
      361 tgacaataaa aacgaattat ctttaaataa atatattcta tttattaacc aagtagctaa
      421 tatagctaaa atcaatttgt tgctaactta aaactaacat aattatgtat tagttattta
      481 cattcttttg gttggttaca accaaataaa taatatcact tttaaaaact taagccagaa
      541 ctgaataatt tctatttggc tttagtcgac ggtatcgata agcttctgaa taagccctcg
      601 taatatattt tcatgaagaa tttaggtcca aaaaaaagat gggcattaat tctagtcatt
      661 taaaaaattc tatagatcag aggttacatg gccaagattg aaacttagag gagtatagtt
      721 acataaaaga aggcaaaacg atgtataaat gaaagaaatt gagatggtgc acgatgcaca
      781 gttgaagtga acttgcgggg tttttcagta tctacgattc attttattaa ttaacctagg
      841 ctactccttc tttgg
//
