LOCUS       sequence-149085-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway(TM) destination vector encoding C-terminal fusion with Venus
            fluorescent protein fragment 1 (V1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Croucher DR, Iconomou M, Hastings JF, Kennedy SP, Han JZ, Shearer 
            RF, McKenna J, Wan A, Lau J, Aparicio S, Saunders DN
  TITLE     Bimolecular complementation affinity purification (BiCAP) reveals 
            dimer-specific protein interactions for ERBB2 dimers.
  JOURNAL   Sci Signal. 2016 Jul 12;9(436):ra69. doi: 10.1126/scisignal.aaf0793.
  PUBMED    27405979
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-07-12"; volume: "9(436):ra69. doi"; pages: " 
            10.1126/scisignal.aaf0793"
COMMENT     Sequence Label: pDEST-ORF-V1
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(99..105,106..123,124..129))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(154..278)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(338..357)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(339..357)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(402..605)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(431..451)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        606..985
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
       61 tcctgaaaat ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata
      121 aagtgtaaag cctggggtgc ctaatgcggc cgccatagtg actggatatg ttgtgtttta
      181 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
      241 ttttacgttt ctcgttcagc ttttttgtac aaacttgtct cgagtacccc gtaatatggt
      301 accaagctta agtttaaacg ctagccagct tgggtctccc tatagtgagt cgtattaatt
      361 tcgataagcc agtaagcagt gggttctcta gttagccaga gagctctgct tatatagacc
      421 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
      481 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
      541 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc
      601 atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
      661 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
      721 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
      781 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
      841 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
      901 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
      961 taataactag tcaataatca atgtcaacgc gtatatctgg cccgtacatc gcga
//
