LOCUS       sequence-149096-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2793 is a vector for multiple integrations at sites sharing 
            homology with Ty2Cons. The selective marker is Kl.URA3. Additionally
            it contains a PTEF1-GFP as reporter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2793
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             85..798
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEKRDHMVLHE
                     YVNAAGITHGMDELYK"
     primer_bind     complement(115..143)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttacaacttt ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt
       61 taattacaaa tctgtcataa aacaatgagc aaaggagaag aacttttcac tggagttgtc
      121 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagaggagag
      181 ggtgaaggtg atgctacaat cggaaaactc acccttaaat ttatttgcac tactggaaaa
      241 ctacctgttc catggccaac acttgtcact actctgacct atggtgttca atgcttttcc
      301 cgttatccgg atcacatgaa aaggcatgac tttttcaaga gtgccatgcc cgaaggttat
      361 gtacaggaac gcactatatc tttcaaagat gacgggaaat acaagacgcg tgctgtagtc
      421 aagtttgaag gtgataccct tgttaatcgt atcgagttaa agggtactga ttttaaagaa
      481 gatggaaaca ttctcggaca caaactcgag tacaacttta actcacacaa tgtatacatc
      541 acggcagaca aacaaaagaa tggaatcaaa gctaacttca cagttcgcca caacgttgaa
      601 gatggttccg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
      661 gtccttttac cagacaacca ttacctgtcg acacaaactg tcctttcgaa agatcccaac
      721 gaaaagcgtg accacatggt ccttcatgag tacgtaaatg ctgctgggat tacacatggc
      781 atggatgagc tctacaaatg aatcgcgtgc attcatccgc tctaaccgaa aaggaaggag
      841 ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa
      901 cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca
      961 ttatactg
//
