LOCUS       sequence-149097-        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Construct for expressing Grb2 lacking the C-terminal SH3 domain in 
            E. Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Su X, Ditlev JA, Hui E, Xing W, Banjade S, Okrut J, King DS, Taunton
            J, Rosen MK, Vale RD
  TITLE     Phase separation of signaling molecules promotes T cell receptor 
            signal transduction.
  JOURNAL   Science. 2016 Apr 29;352(6285):595-9. doi: 10.1126/science.aad9964. 
            Epub 2016 Apr 7.
  PUBMED    27056844
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad9964"; journalName: "Science"; date: 
            "2016-04-29- 29"; volume: "352"; issue: "6285"; pages: "595-9"
COMMENT     Sequence Label: GST-PS-Grb2 1-154 (XSB382)
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(205..309)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     377..395
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     540..562
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
       61 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
      121 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
      181 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
      241 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
      301 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
      361 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattatacac
      421 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
      481 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
      541 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagatcgt
      601 cagtcagtca cgatgcggcc gctcgagtcg acttacacct gttctatgtc ccgcaggaat
      661 atctgctggt ttctggagac agatgtagat ctgtgataat ccaccagctc attcaaagaa
      721 ttgaacttca ccacccagag gaagtacttc ccggctccat ctcggagcac cttgaagtgc
      781 tgcacatcgt ttccaaactt gacagagagg gagaagtccc caggagcgct ctcactctct
      841 cggataagaa aggccccatc gtgccgctgt ttgctaagca tttcttctgc cttggctctg
      901 gggattttgc caaaaaacca cggatgtggt ttcatttcta tgtagttctt gggaatgaag
      961 ccgtcttttc cattaagctc tgccttgtac cagttctgat cacattcttc gttcaaaacc
     1021 ttgaggatgt cccccctttt gaagctcagc tcgtcgtctg cagtagcttt gaagtcatat
     1081 ttg
//
