LOCUS       sequence-149098-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Construct for expressing Grb2 lacking the C-terminal SH3 domain in 
            E. Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Su X, Ditlev JA, Hui E, Xing W, Banjade S, Okrut J, King DS, Taunton
            J, Rosen MK, Vale RD
  TITLE     Phase separation of signaling molecules promotes T cell receptor 
            signal transduction.
  JOURNAL   Science. 2016 Apr 29;352(6285):595-9. doi: 10.1126/science.aad9964. 
            Epub 2016 Apr 7.
  PUBMED    27056844
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad9964"; journalName: "Science"; date: 
            "2016-04-29- 29"; volume: "352"; issue: "6285"; pages: "595-9"
COMMENT     Sequence Label: GST-PS-Grb2 1-154 (XSB382)
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(42..47,48..63,64..70)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    78..94
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     83..105
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             117..770
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     728..750
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             777..800
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 cgacatcata acggttctgg caaatattct gaaatgagct gttgacaatt aatcatcggc
       61 tcgtataatg tgtggaattg tgagcggata acaatttcac acaggaaaca gtattcatgt
      121 cccctatact aggttattgg aaaattaagg gccttgtgca acccactcga cttcttttgg
      181 aatatcttga agaaaaatat gaagagcatt tgtatgagcg cgatgaaggt gataaatggc
      241 gaaacaaaaa gtttgaattg ggtttggagt ttcccaatct tccttattat attgatggtg
      301 atgttaaatt aacacagtct atggccatca tacgttatat agctgacaag cacaacatgt
      361 tgggtggttg tccaaaagag cgtgcagaga tttcaatgct tgaaggagcg gttttggata
      421 ttagatacgg tgtttcgaga attgcatata gtaaagactt tgaaactctc aaagttgatt
      481 ttcttagcaa gctacctgaa atgctgaaaa tgttcgaaga tcgtttatgt cataaaacat
      541 atttaaatgg tgatcatgta acccatcctg acttcatgtt gtatgacgct cttgatgttg
      601 ttttatacat ggacccaatg tgcctggatg cgttcccaaa attagtttgt tttaaaaaac
      661 gtattgaagc tatcccacaa attgataagt acttgaaatc cagcaagtat atagcatggc
      721 ctttgcaggg ctggcaagcc acgtttggtg gtggcgacca tcctccaaaa tcggatctgg
      781 aagttctgtt ccaggggccc ctgggatcca tggaagccat cgccaaatat gacttcaaag
      841 ctactgcaga cgacgagctg agcttcaaaa ggggggacat cctcaaggtt ttgaacgaag
      901 aatgtgatca gaactggtac aaggcagagc ttaatggaaa agacggcttc attcccaaga
      961 actac
//
