LOCUS       sequence-149099-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2793 is a vector for multiple integrations at sites sharing 
            homology with Ty2Cons. The selective marker is Kl.URA3. Additionally
            it contains a PTEF1-GFP as reporter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2793
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      59..248
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    296..329
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aatgctgctg ggattacaca tggcatggat gagctctaca aatgaatcgc gtgcattcat
       61 ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt
      121 ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt
      181 acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac
      241 gctcgaagat cgcgtcagct gaagcttcgt acgctgcagg tcgacaaccc ttaatataac
      301 ttcgtataat gtatgctata cgaagttatt aggtctagag atcccaatac aacagatcac
      361 gtgatctttt tgtaagatga agttgaagta gtgttgcacc gtgccaatgc aggtggctat
      421 tagattaaat atgtgatttg ttctattaag tttcctgtat aattacaaat gaataacgaa
      481 atgagacaaa gaagagaacc aatttttaca agcatgggga gcgctgattc tcttttggta
      541 cgcttcccat ccagcatttc tgtatctttc accttcaacc ttaggatctc tacccttggc
      601 gaaaagtcct ctgccaacaa tgatgatatc tgatccacca cttacaactt cgtcgacggt
      661 tctgtactgc tgacccaatg catcgccttt gtcgtctaaa cctacacctg gggtcatgat
      721 tagccaatca aacccttctt ctcttcctcc catatcgttc tgagcaatga acccaataac
      781 gaaatcttta tcactctttg caatatcaac ggtaccctta gtatattcac cgtgtgctag
      841 agaacccttg gaagataatt cagcaagcat caataatccc cttggttctt tggtgacctc
      901 ttgcgcacct tgtttcaagc cagcaacaat accagcacca gtaaccccgt gggcgttggt
      961 gatatcagac cattctgcga tacggtaaa
//
