LOCUS       sequence-149100-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Construct for expressing Gads SH3, SH2, and SH3 domain in E. Coli, 
            with a C-terminal Cys for labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Su X, Ditlev JA, Hui E, Xing W, Banjade S, Okrut J, King DS, Taunton
            J, Rosen MK, Vale RD
  TITLE     Phase separation of signaling molecules promotes T cell receptor 
            signal transduction.
  JOURNAL   Science. 2016 Apr 29;352(6285):595-9. doi: 10.1126/science.aad9964. 
            Epub 2016 Apr 7.
  PUBMED    27056844
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad9964"; journalName: "Science"; date: 
            "2016-04-29- 29"; volume: "352"; issue: "6285"; pages: "595-9"
COMMENT     Sequence Label: GST-PS-Gads 1-155 GB1 Gads 261-330-Cys (XSB365)
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     87..105
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     250..272
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(559..720)
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
ORIGIN
        1 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
       61 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
      121 aattatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
      181 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
      241 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
      301 ggcagatcgt cagtcagtca cgatgcggcc gctcgagtcg acttagcacc gggtcatggg
      361 tgccacgtag ttggcaggga agaggcccag cttgttgtgc aggcggccgg tccaccagga
      421 tgggttggag ctatccagga cctccaccac ctccccgctg tggaacccca gctcgtcatc
      481 ctccagggcc tcaaagtcat acagcgcccg ggcccaccgc actcgccctg ccgcctggag
      541 ctgcactggg tctgtgtgtt cggttaccgt gaaggttttg gtagcgtcgt cgtaggtcca
      601 ttcaccgtca acaccgttgt cgttagcgta ctgtttgaaa actttttccg cggtagcagc
      661 gtcaacagct tcggtggtgg tttcaccttt cagggtttta ccgttcagga taagcttgta
      721 ctcgtgaccc tggtcttctc gggttctgtc tctaaggaag atctgcttct gtctggagat
      781 ggaatttgtc ctgtagtagt ctaccagctt atttagggat ggaaacttct cagtccacag
      841 aaagtaatta cccttgttgt ctcgcatgac cttgaagtgt tgaacgtcat cctcatgcct
      901 gacagagatg gagaagtccc ctggggagct ctggctggcc cggatgatga agaagccaac
      961 ctccttgccc atgagtaagt tctctgcctg gtgtcgagag aggccttcgt gaaaccattt
     1021 gggaaactgg atgtctatga aattcttggg cacatat
//
