LOCUS       sequence-149103-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Construct for expressing Gads SH3, SH2, and SH3 domain in E. Coli, 
            with a C-terminal Cys for labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Su X, Ditlev JA, Hui E, Xing W, Banjade S, Okrut J, King DS, Taunton
            J, Rosen MK, Vale RD
  TITLE     Phase separation of signaling molecules promotes T cell receptor 
            signal transduction.
  JOURNAL   Science. 2016 Apr 29;352(6285):595-9. doi: 10.1126/science.aad9964. 
            Epub 2016 Apr 7.
  PUBMED    27056844
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad9964"; journalName: "Science"; date: 
            "2016-04-29- 29"; volume: "352"; issue: "6285"; pages: "595-9"
COMMENT     Sequence Label: GST-PS-Gads 1-155 GB1 Gads 261-330-Cys (XSB365)
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(51..56,57..72,73..79)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    87..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     92..114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             126..779
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     737..759
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             786..809
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 tttttgcgcc gacatcataa cggttctggc aaatattctg aaatgagctg ttgacaatta
       61 atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag
      121 tattcatgtc ccctatacta ggttattgga aaattaaggg ccttgtgcaa cccactcgac
      181 ttcttttgga atatcttgaa gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg
      241 ataaatggcg aaacaaaaag tttgaattgg gtttggagtt tcccaatctt ccttattata
      301 ttgatggtga tgttaaatta acacagtcta tggccatcat acgttatata gctgacaagc
      361 acaacatgtt gggtggttgt ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg
      421 ttttggatat tagatacggt gtttcgagaa ttgcatatag taaagacttt gaaactctca
      481 aagttgattt tcttagcaag ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc
      541 ataaaacata tttaaatggt gatcatgtaa cccatcctga cttcatgttg tatgacgctc
      601 ttgatgttgt tttatacatg gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt
      661 ttaaaaaacg tattgaagct atcccacaaa ttgataagta cttgaaatcc agcaagtata
      721 tagcatggcc tttgcagggc tggcaagcca cgtttggtgg tggcgaccat cctccaaaat
      781 cggatctgga agttctgttc caggggcccc tgggatccat ggaagctgtt gccaagtttg
      841 atttcactgc ttcaggtgag gatgaactga gctttcacac tggagatgtt ttgaagattt
      901 taagtaacca agaggagtgg tttaaggcgg agcttgggag ccaggaagga tatgtgccca
      961 agaatttcat agacatccag tttcccaaat ggtttcacga aggcctctct cgaca
//
