LOCUS       sequence-149104-        1094 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Construct for expressing Sos1 proline-rich motif in E. Coli, with a 
            C-terminal Cys for labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Su X, Ditlev JA, Hui E, Xing W, Banjade S, Okrut J, King DS, Taunton
            J, Rosen MK, Vale RD
  TITLE     Phase separation of signaling molecules promotes T cell receptor 
            signal transduction.
  JOURNAL   Science. 2016 Apr 29;352(6285):595-9. doi: 10.1126/science.aad9964. 
            Epub 2016 Apr 7.
  PUBMED    27056844
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad9964"; journalName: "Science"; date: 
            "2016-04-29- 29"; volume: "352"; issue: "6285"; pages: "595-9"
COMMENT     Sequence Label: GST-PS-Sos1 (PRM) - Cys (XSB364)
FEATURES             Location/Qualifiers
     source          1..1094
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     92..110
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     255..277
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(972..995)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(1022..1044)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
       61 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
      121 tcaagaatta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
      181 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
      241 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
      301 cgcgaggcag atcgtcagtc agtcacgatg cggccgctcg agtcgactta gcacatggat
      361 gggtgtgtgt gctcacgctt atatgttttc ggcggaagtt tcgggatatg ctgcgaggta
      421 ctctggcgtg gcggcaccgg aggacccgcg atactgtgca gatcgacttc ctgggtcagc
      481 ggcgggctgg ggagatgacg ccgagtcccg tgcggcgaag gggtttgcgg cgggggcggc
      541 gtaaacggcg atggcgagtt tggaaagaac gcattcccgt gatcagattt cttgcccagc
      601 ggtggcggtt gcagatgcaa cggactgcta gagaacacat ccggtgtgcg aaccggttcg
      661 cgcggcggca gcagcggggg cgattctggt gggtcagaaa ttgacgtacg atctgaaatg
      721 ctgtagcgtg gactatatgc tttgctggtc ggttgacgcg gcgggattgc gggcggacta
      781 tccagatgtt tgctcatgat tttacttggg gaagattctg ctggggctga ttctgggcgg
      841 cgacgcggag gcactggcgg cggaacaggt acttcatcgg tacctttggt cagactgatg
      901 ctgctcacgg aggcagaacg gggaccgtgc ggcagagtaa cttggataaa gacggtatca
      961 ttggatccca ggggcccctg gaacagaact tccagatccg attttggagg atggtcgcca
     1021 ccaccaaacg tggcttgcca gccctgcaaa ggccatgcta tatacttgct ggatttcaag
     1081 tacttatcaa tttg
//
