LOCUS       sequence-149106-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Construct for expressing Sos1 proline-rich motif in E. Coli, with a 
            C-terminal Cys for labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Su X, Ditlev JA, Hui E, Xing W, Banjade S, Okrut J, King DS, Taunton
            J, Rosen MK, Vale RD
  TITLE     Phase separation of signaling molecules promotes T cell receptor 
            signal transduction.
  JOURNAL   Science. 2016 Apr 29;352(6285):595-9. doi: 10.1126/science.aad9964. 
            Epub 2016 Apr 7.
  PUBMED    27056844
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad9964"; journalName: "Science"; date: 
            "2016-04-29- 29"; volume: "352"; issue: "6285"; pages: "595-9"
COMMENT     Sequence Label: GST-PS-Sos1 (PRM) - Cys (XSB364)
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(54..59,60..75,76..82)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    90..106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     95..117
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             129..782
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     740..762
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             789..812
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa
       61 ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
      121 cagtattcat gtcccctata ctaggttatt ggaaaattaa gggccttgtg caacccactc
      181 gacttctttt ggaatatctt gaagaaaaat atgaagagca tttgtatgag cgcgatgaag
      241 gtgataaatg gcgaaacaaa aagtttgaat tgggtttgga gtttcccaat cttccttatt
      301 atattgatgg tgatgttaaa ttaacacagt ctatggccat catacgttat atagctgaca
      361 agcacaacat gttgggtggt tgtccaaaag agcgtgcaga gatttcaatg cttgaaggag
      421 cggttttgga tattagatac ggtgtttcga gaattgcata tagtaaagac tttgaaactc
      481 tcaaagttga ttttcttagc aagctacctg aaatgctgaa aatgttcgaa gatcgtttat
      541 gtcataaaac atatttaaat ggtgatcatg taacccatcc tgacttcatg ttgtatgacg
      601 ctcttgatgt tgttttatac atggacccaa tgtgcctgga tgcgttccca aaattagttt
      661 gttttaaaaa acgtattgaa gctatcccac aaattgataa gtacttgaaa tccagcaagt
      721 atatagcatg gcctttgcag ggctggcaag ccacgtttgg tggtggcgac catcctccaa
      781 aatcggatct ggaagttctg ttccaggggc ccctgggatc caatgatacc gtctttatcc
      841 aagttactct gccgcacggt ccccgttctg cctccgtgag cagcatcagt ctgaccaaag
      901 gtaccgatga agtacctgtt ccgccgccag tgcctccgcg tcgccgccca gaatcagccc
      961 cagcagaatc ttccccaagt aaaatcatga gcaa
//
