LOCUS       sequence-149108-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Methionine Binding Protein (MetQ) with C-Terminal 6x Histidine Tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Nguyen PT, Li QW, Kadaba NS, Lai JY, Yang JG, Rees DC
  TITLE     The contribution of methionine to the stability of the Escherichia 
            coli MetNIQ ABC transporter-substrate binding protein complex.
  JOURNAL   Biol Chem. 2015 Sep;396(9-10):1127-34. doi: 10.1515/hsz-2015-0131.
  PUBMED    25803078
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Chem."; date: "2015-09"; volume: "396(9-10):1127-34. doi"; pages: " 
            10.1515/hsz-2015-0131"
COMMENT     Sequence Label: Lf-MetQ
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(1..77)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     283..302
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     386..405
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        386..404
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    405..429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             444..466
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
       61 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
      121 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
      181 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
      241 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
      301 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
      361 gatcgagatc tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat
      421 aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata catatggcgt
      481 tcaaattcaa aacctttgcg gcagtgggag ccctgatcgg atcactggca ctggtaggct
      541 gcggtcagga tgaaaaagat ccaaaccaca ttaaagtcgg cgtgattgtt ggtgccgaac
      601 agcaggttgc agaagtcgcg cagaaagttg cgaaagacaa atatggcctg gacgttgagc
      661 tggtaacctt caacgactat gttctgccaa acgaagcatt gagcaaaggc gatatcgacg
      721 ccaacgcctt ccagcataaa ccgtaccttg atcagcaact gaaagatcgt ggctacaaac
      781 tggtcgcagt aggcaacact tttgtttatc cgattgctgg ttactccaag aaaatcaaat
      841 cactggatga actgcaggat ggttcgcagg ttgccgtgcc aaacgaccca actaaccttg
      901 gtcgttcact gctgctgctg caaaaagtgg gcttgatcaa actgaaagat ggcgttggcc
      961 tgctgccgac cgttcttgat gttgttgaga accccaaaaa tctgaaaatt gttgaactgg
     1021 aagcaccgca actgccgcgt tctctgg
//
