LOCUS       sequence-149109-        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Methionine ABC Transporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Johnson E, Nguyen PT, Yeates TO, Rees DC
  TITLE     Inward facing conformations of the MetNI methionine ABC transporter:
            Implications for the mechanism of transinhibition.
  JOURNAL   Protein Sci. 2012 Jan;21(1):84-96. doi: 10.1002/pro.765. Epub 2011 
            Dec 5.
  PUBMED    22095702
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.765"; 
            journalName: "Protein Sci"; date: "2012-01"; volume: "21"; issue: 
            "1"; pages: "84-96"
COMMENT     Sequence Label: MetNI (E166Q in MetN)
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..20
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(47..52,53..69,70..75)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(250..297)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     293..311
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 aaataggcgt atcacgaggc cctttcgtct tcaagaattc tcatgtttga cagcttatca
       61 tcgataagct ttaatgcggt agtttatcac agttaaattg ctaacgcagt caggcaccgt
      121 gtatgaaatc taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag
      181 gcataggctt ggttatgccg gtactgccgg gcctcttgcg ggatatccgg atatagttcc
      241 tcctttcagc aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt atgctagtta
      301 ttgctcagcg gtggcagcag ccaactcagc ttcctttcgg gctttgttag cagccggatc
      361 ctcgagtcag acataaccca gtacctctac ttttacatgg tgttcctgca gccaggcaat
      421 ggcggcttgc gtatcttgtt gtgtgccgtg catttcagtc agcatgatgc cgaacttaac
      481 gccaccggcg taatccatct gcgcgctaat aatgttgttg ttgacgttga aacgacgcgc
      541 ggtttcagaa agcagtgggg catcgaccga ttgaccggta aactccagac gcagcatcgg
      601 cacgcagtca gtaaatggct ccgcttgcag acgttcctgg taatcttccg ggatatccag
      661 atgcagggtc gactgaataa acttctgcgc cagcggcgtt ttcggatgcg agaacacttc
      721 acttaccgtg tcctgctcga tcagttctcc attgctgatg accgccacgc aatcacaaat
      781 gcgcttcaca acgtccattt cgtgggtgat caacagaatc gtcaacccca gacggcggtt
      841 gatgtctttc agcagttcga gaatagaacg tgtcgttgcc gggtccagcg cgctggtggc
      901 ttgatcacac agcaatactt tgggattgct ggctaacgca cgggcaattg ccacacgttg
      961 tttctgccca ccggaaagat tcgacgggta gctatcatgc ttatcgccaa gaccaaccaa
     1021 tgacagcaat tccgtcacgc gacgtttgac ctcgtctttc ggtgtgttgt ccagctccag
     1081 cg
//
