LOCUS       sequence-149110-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Methionine ABC Transporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Johnson E, Nguyen PT, Yeates TO, Rees DC
  TITLE     Inward facing conformations of the MetNI methionine ABC transporter:
            Implications for the mechanism of transinhibition.
  JOURNAL   Protein Sci. 2012 Jan;21(1):84-96. doi: 10.1002/pro.765. Epub 2011 
            Dec 5.
  PUBMED    22095702
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.765"; 
            journalName: "Protein Sci"; date: "2012-01"; volume: "21"; issue: 
            "1"; pages: "84-96"
COMMENT     Sequence Label: MetNI (E166Q in MetN)
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(2..79)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     195..214
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        195..213
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    214..238
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             253..275
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             940..957
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1010..1028)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
       61 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
      121 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
      181 cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc
      241 tctagaaata attttgttta actttaagaa ggagatatac atatgtctga gccgatgatg
      301 tggctgctgg ttcgtggcgt atgggaaacg ctggcaatga ccttcgtatc cggttttttt
      361 ggctttgtga ttggtctgcc ggttggcgtt ctgctttatg tcacgcgtcc ggggcaaatt
      421 attgctaacg cgaagctgta tcgtaccgtt tctgcgattg tgaacatttt ccgttccatc
      481 ccgttcatta tcttgcttgt atggatgatt ccgtttaccc gcgttattgt cggtacatcg
      541 attggtttgc aggcagcgat tgttccgtta accgttggtg cagcaccgtt tattgcccgt
      601 atggtcgaga acgctctgct ggagatccca accgggttaa ttgaagcttc ccgcgcaatg
      661 ggtgccacgc cgatgcagat cgtccgtaag gtgctgttac cggaagcgct gccgggtctg
      721 gtgaatgcgg caactatcac cctgattacc ctggtcggtt attccgcgat gggtggtgca
      781 gtcggtgccg gtggtttagg tcagattggc tatcagtatg gctacatcgg ctataacgcg
      841 acggtgatga atacggtact ggtattgctg gtcattctgg tttatttaat tcagttcgca
      901 ggcgaccgca tcgtccgggc tgtcactcgc aagtgagagc accaccacca ccaccactga
      961 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     1021 taactagcat aacccc
//
