LOCUS       sequence-149116-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To purify His-tagged recombinant FADD protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  TITLE     Song lab plasmids
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET32a-His-FADD
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             35..361
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             383..400
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             410..427
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             434..478
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             494..508
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgagc gataaaatta ttcacctgac
       61 tgacgacagt tttgacacgg atgtactcaa agcggacggg gcgatcctcg tcgatttctg
      121 ggcagagtgg tgcggtccgt gcaaaatgat cgccccgatt ctggatgaaa tcgctgacga
      181 atatcagggc aaactgaccg ttgcaaaact gaacatcgat caaaaccctg gcactgcgcc
      241 gaaatatggc atccgtggta tcccgactct gctgctgttc aaaaacggtg aagtggcggc
      301 aaccaaagtg ggtgcactgt ctaaaggtca gttgaaagag ttcctcgacg ctaacctggc
      361 cggttctggt tctggccata tgcaccatca tcatcatcat tcttctggtc tggtgccacg
      421 cggttctggt atgaaagaaa ccgctgctgc taaattcgaa cgccagcaca tggacagccc
      481 agatctgggt accgacgacg acgacaaggc catggctgat atcggatccg aattcatgga
      541 cccgttcctg gtgctgctgc actcggtgtc gtccagcctg tcgagcagcg agctgaccga
      601 gctcaagttc ctatgcctcg ggcgcgtggg caagcgcaag ctggagcgcg tgcagagcgg
      661 cctagacctc ttctccatgc tactggagca gaacgacctg gagcccgggc acaccgagct
      721 cctgcgcgag ctgctcgcct ccctgcggcg ccacgacctg ctgcggcgcg tcgacgactt
      781 cgaggcgggg gcggcggccg gggccgcgcc tggggaagaa ga
//
