LOCUS       sequence-149117-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress FADD in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Lee EW, Kim JH, Ahn YH, Seo J, Ko A, Jeong M, Kim SJ, Ro JY, Park 
            KM, Lee HW, Park EJ, Chun KH, Song J
  TITLE     Ubiquitination and degradation of the FADD adaptor protein regulate 
            death receptor-mediated apoptosis and necroptosis.
  JOURNAL   Nat Commun. 2012;3:978. doi: 10.1038/ncomms1981.
  PUBMED    22864571
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:978. doi"; pages: " 
            10.1038/ncomms1981"
COMMENT     Sequence Label: pcDNA3-FLAG-FADD
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      71..80
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             80..103
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(779..797)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(780..797)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(817..834)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgcc gccaccatgg attacaagga tgacgacgat aagagcgcgg gcggatccga
      121 attcatggac ccgttcctgg tgctgctgca ctcggtgtcg tccagcctgt cgagcagcga
      181 gctgaccgag ctcaagttcc tatgcctcgg gcgcgtgggc aagcgcaagc tggagcgcgt
      241 gcagagcggc ctagacctct tctccatgct actggagcag aacgacctgg agcccgggca
      301 caccgagctc ctgcgcgagc tgctcgcctc cctgcggcgc cacgacctgc tgcggcgcgt
      361 cgacgacttc gaggcggggg cggcggccgg ggccgcgcct ggggaagaag acctgtgtgc
      421 agcatttaac gtcatatgtg ataatgtggg gaaagattgg agaaggctgg ctcgtcagct
      481 caaagtctca gacaccaaga tcgacagcat cgaggacaga tacccccgca acctgacaga
      541 gcgtgtgcgg gagtcactga gaatctggaa gaacacagag aaggagaacg caacagtggc
      601 ccacctggtg ggggctctca ggtcctgcca gatgaacctg gtggctgacc tggtacaaga
      661 ggttcagcag gcccgtgacc tccagaacag gagtggggcc atgtccccga tgtcatggaa
      721 ctcagacgca tctacctccg aagcgtcctg actcgagcat gcatctagag ggccctattc
      781 tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc
      841 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctgga
//
