LOCUS       sequence-149118-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress FADD in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Lee EW, Kim JH, Ahn YH, Seo J, Ko A, Jeong M, Kim SJ, Ro JY, Park 
            KM, Lee HW, Park EJ, Chun KH, Song J
  TITLE     Ubiquitination and degradation of the FADD adaptor protein regulate 
            death receptor-mediated apoptosis and necroptosis.
  JOURNAL   Nat Commun. 2012;3:978. doi: 10.1038/ncomms1981.
  PUBMED    22864571
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:978. doi"; pages: " 
            10.1038/ncomms1981"
COMMENT     Sequence Label: pcDNA3-FLAG-FADD
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(683..706)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      706..715
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(731..750)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(732..750)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagtcagga cgcttcggag gtagatgcgt
       61 ctgagttcca tgacatcggg gacatggccc cactcctgtt ctggaggtca cgggcctgct
      121 gaacctcttg taccaggtca gccaccaggt tcatctggca ggacctgaga gcccccacca
      181 ggtgggccac tgttgcgttc tccttctctg tgttcttcca gattctcagt gactcccgca
      241 cacgctctgt caggttgcgg gggtatctgt cctcgatgct gtcgatcttg gtgtctgaga
      301 ctttgagctg acgagccagc cttctccaat ctttccccac attatcacat atgacgttaa
      361 atgctgcaca caggtcttct tccccaggcg cggccccggc cgccgccccc gcctcgaagt
      421 cgtcgacgcg ccgcagcagg tcgtggcgcc gcagggaggc gagcagctcg cgcaggagct
      481 cggtgtgccc gggctccagg tcgttctgct ccagtagcat ggagaagagg tctaggccgc
      541 tctgcacgcg ctccagcttg cgcttgccca cgcgcccgag gcataggaac ttgagctcgg
      601 tcagctcgct gctcgacagg ctggacgaca ccgagtgcag cagcaccagg aacgggtcca
      661 tgaattcgga tccgcccgcg ctcttatcgt cgtcatcctt gtaatccatg gtggcggcaa
      721 gcttgggtct ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct
      781 ctagt
//
