LOCUS       sequence-149127-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress p21 K163R in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Lee MS, Seo J, Choi DY, Lee EW, Ko A, Ha NC, Yoon JB, Lee HW, Kim 
            KP, Song J
  TITLE     Stabilization of p21 (Cip1/WAF1) following Tip60-dependent 
            acetylation is required for p21-mediated DNA damage response.
  JOURNAL   Cell Death Differ. 2013 Apr;20(4):620-9. doi: 10.1038/cdd.2012.159. 
            Epub 2012 Dec 14.
  PUBMED    23238566
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cdd.2012.159"; journalName: "Cell Death Differ"; date: 
            "2013-04"; volume: "20"; issue: "4"; pages: "620-9"
COMMENT     Sequence Label: pcDNA3-HA p21 K163R
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..529)
                     /codon_start=1
                     /gene="Homo sapiens CDKN1A"
                     /product="human cyclin-dependent kinase inhibitor 1A"
                     /label=p21
                     /note="mediates p53-dependent arrest in the G1 phase of the
                     cell cycle"
                     /translation="MSEPAGDVRQNPCGSKACRRLFGPVDSEQLRRDCDALMAGCIQEA
                     RERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQG
                     TAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRR
                     P"
     CDS             complement(542..568)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(596..615)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(597..615)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(660..863)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(689..709)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagttaggg cctcctcttg gagaagatca
       61 gccggcgttt ggagtggtag aaatctgtca tgctggtctg ccgccgtttt cgaccctgag
      121 agtctccagg tccacctggg gacccttcag cctgctcccc tgagcgaggc acaagggtac
      181 aagacagtga caggtccaca tggtcttcct ctgctgtccc ctgcagcaga gcaggtgagg
      241 tgccaggccg cctgcctcct cccaactcat cccggcctcg ccggggcccc gtgggaaggt
      301 agagcttggg caggccaagg ccccgcacac gctcccaggc gaagtcaccc tccagtggtg
      361 tctcggtgac aaagtcgaag ttccatcgct cacgggcctc ctggatgcag cccgccatta
      421 gcgcatcaca gtcgcgtctc agctgctcgc tgtccactgg gccgaagagg cggcggcagg
      481 ccttgctgcc gcatgggttc tgacggacat ccccagccgg ttctgacatg aattcgggag
      541 aggcataatc tggcacatca taagggtagg aggccatggt accaagcttg ggtctcccta
      601 tagtgagtcg tattaatttc gataagccag taagcagtgg gttctctagt tagccagaga
      661 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
      721 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
      781 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
      841 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
//
