LOCUS       sequence-149131-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress p21 K161R in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Lee MS, Seo J, Choi DY, Lee EW, Ko A, Ha NC, Yoon JB, Lee HW, Kim 
            KP, Song J
  TITLE     Stabilization of p21 (Cip1/WAF1) following Tip60-dependent 
            acetylation is required for p21-mediated DNA damage response.
  JOURNAL   Cell Death Differ. 2013 Apr;20(4):620-9. doi: 10.1038/cdd.2012.159. 
            Epub 2012 Dec 14.
  PUBMED    23238566
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cdd.2012.159"; journalName: "Cell Death Differ"; date: 
            "2013-04"; volume: "20"; issue: "4"; pages: "620-9"
COMMENT     Sequence Label: pcDNA3-HA p21 K161R
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             100..126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             139..633
                     /codon_start=1
                     /gene="Homo sapiens CDKN1A"
                     /product="human cyclin-dependent kinase inhibitor 1A"
                     /label=p21
                     /note="mediates p53-dependent arrest in the G1 phase of the
                     cell cycle"
                     /translation="MSEPAGDVRQNPCGSKACRRLFGPVDSEQLRRDCDALMAGCIQEA
                     RERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQG
                     TAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSRRK
                     P"
     promoter        complement(661..679)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(662..679)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(699..716)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    705..816
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc atggcctcct acccttatga tgtgccagat
      121 tatgcctctc ccgaattcat gtcagaaccg gctggggatg tccgtcagaa cccatgcggc
      181 agcaaggcct gccgccgcct cttcggccca gtggacagcg agcagctgag acgcgactgt
      241 gatgcgctaa tggcgggctg catccaggag gcccgtgagc gatggaactt cgactttgtc
      301 accgagacac cactggaggg tgacttcgcc tgggagcgtg tgcggggcct tggcctgccc
      361 aagctctacc ttcccacggg gccccggcga ggccgggatg agttgggagg aggcaggcgg
      421 cctggcacct cacctgctct gctgcagggg acagcagagg aagaccatgt ggacctgtca
      481 ctgtcttgta cccttgtgcc tcgctcaggg gagcaggctg aagggtcccc aggtggacct
      541 ggagactctc agggtcgaaa acggcggcag accagcatga cagatttcta ccactccaaa
      601 cgccggctga tcttctccag gaggaagccc taactcgagc atgcatctag agggccctat
      661 tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
      721 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
      781 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
      841 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagca
//
