LOCUS       sequence-149149-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of p21.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Lee MS, Seo J, Choi DY, Lee EW, Ko A, Ha NC, Yoon JB, Lee HW, Kim 
            KP, Song J
  TITLE     Stabilization of p21 (Cip1/WAF1) following Tip60-dependent 
            acetylation is required for p21-mediated DNA damage response.
  JOURNAL   Cell Death Differ. 2013 Apr;20(4):620-9. doi: 10.1038/cdd.2012.159. 
            Epub 2012 Dec 14.
  PUBMED    23238566
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cdd.2012.159"; journalName: "Cell Death Differ"; date: 
            "2013-04"; volume: "20"; issue: "4"; pages: "620-9"
COMMENT     Sequence Label: pBABE-puro p21
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..517
                     /codon_start=1
                     /gene="Homo sapiens CDKN1A"
                     /product="human cyclin-dependent kinase inhibitor 1A"
                     /label=p21
                     /note="mediates p53-dependent arrest in the G1 phase of the
                     cell cycle"
                     /translation="MSEPAGDVRQNPCGSKACRRLFGPVDSEQLRRDCDALMAGCIQEA
                     RERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQG
                     TAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRK
                     P"
     primer_bind     complement(529..549)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        547..844
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      714..849
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gtgtggtggt acgtaggaat tcatgtcaga accggctggg gatgtccgtc agaacccatg
       61 cggcagcaag gcctgccgcc gcctcttcgg cccagtggac agcgagcagc tgagacgcga
      121 ctgtgatgcg ctaatggcgg gctgcatcca ggaggcccgt gagcgatgga acttcgactt
      181 tgtcaccgag acaccactgg agggtgactt cgcctgggag cgtgtgcggg gccttggcct
      241 gcccaagctc taccttccca cggggccccg gcgaggccgg gatgagttgg gaggaggcag
      301 gcggcctggc acctcacctg ctctgctgca ggggacagca gaggaagacc atgtggacct
      361 gtcactgtct tgtacccttg tgcctcgctc aggggagcag gctgaagggt ccccaggtgg
      421 acctggagac tctcagggtc gaaaacggcg gcagaccagc atgacagatt tctaccactc
      481 caaacgccgg ctgatcttct ccaagaggaa gccctaactc gaccctgtgg aatgtgtgtc
      541 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
      601 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
      661 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
      721 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
      781 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
      841 ttggaggcct a
//
