LOCUS       sequence-149161-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress RIPK1 in Mammalian Cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee 
            HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J
  TITLE     CHIP controls necroptosis through ubiquitylation- and 
            lysosome-dependent degradation of RIPK3.
  JOURNAL   Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 
            2016 Feb 22.
  PUBMED    26900751
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3314"; 
            journalName: "Nat Cell Biol"; date: "2016-03"; volume: "18"; issue: 
            "3"; pages: "291-302"
COMMENT     Sequence Label: pcDNA3-HA-RIPK1
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             97..123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccatg gcctcctacc cttatgatgt gccagattat
      121 gcctctcccg aattcatgca accagacatg tccttgaatg tcattaagat gaaatccagt
      181 gacttcctgg agagtgcaga actggacagc ggaggctttg ggaaggtgtc tctgtgtttc
      241 cacagaaccc agggactcat gatcatgaaa acagtgtaca aggggcccaa ctgcattgag
      301 cacaacgagg ccctcttgga ggaggcgaag atgatgaaca gactgagaca cagccgggtg
      361 gtgaagctcc tgggcgtcat catagaggaa gggaagtact ccctggtgat ggagtacatg
      421 gagaagggca acctgatgca cgtgctgaaa gccgagatga gtactccgct ttctgtaaaa
      481 ggaaggataa ttttggaaat cattgaagga atgtgctact tacatggaaa aggcgtgata
      541 cacaaggacc tgaagcctga aaatatcctt gttgataatg acttccacat taagatcgca
      601 gacctcggcc ttgcctcctt taagatgtgg agcaaactga ataatgaaga gcacaatgag
      661 ctgagggaag tggacggcac cgctaagaag aatggcggca ccctctacta catggcgccc
      721 gagcacctga atgacgtcaa cgcaaagccc acagagaagt cggatgtgta cagctttgct
      781 gtagtactct gggcgatatt tgcaaataag gagccatatg aaaatgctat ctgtgagcag
      841 cagttgataa tgtgcataa
//
