LOCUS       sequence-149164-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Derived from 21 with silent mutations to make BsaI, BbsI compatible 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Sun ZZ, Yeung E, Hayes CA, Noireaux V, Murray RM
  TITLE     Linear DNA for rapid prototyping of synthetic biological circuits in
            an Escherichia coli based TX-TL cell-free system.
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. 
            Epub 2013 Dec 4.
  PUBMED    24303785
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400131a";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "387-97"
COMMENT     Sequence Label: pBEST_OR2-OR1-Pr_UTR1-deGFP-T500
            BsaI, BbsI-sa...
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             142..164
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(201..222)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(462..481)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     809..830
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgatatccg cctgatgcgt gaacgtgacg gacgtaacca ccgcgacatg tgtgtgctgt
       61 tccgctgggc atgctgagct aacaccgtgc gtgttgacaa ttttacctct ggcggtgata
      121 atggttgcag ctagcaataa ttttgtttaa ctttaagaag gagatatacc atggagcttt
      181 tcactggcgt tgttcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      241 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      301 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
      361 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      421 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      481 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      541 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      601 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
      661 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
      721 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
      781 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
      841 ggatctaact cgagca
//
