LOCUS       sequence-149165-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Derived from 21 with silent mutations to make BsaI, BbsI compatible 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Sun ZZ, Yeung E, Hayes CA, Noireaux V, Murray RM
  TITLE     Linear DNA for rapid prototyping of synthetic biological circuits in
            an Escherichia coli based TX-TL cell-free system.
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. 
            Epub 2013 Dec 4.
  PUBMED    24303785
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400131a";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "387-97"
COMMENT     Sequence Label: pBEST_OR2-OR1-Pr_UTR1-deGFP-T500
            BsaI, BbsI-sa...
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(145..166)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     494..513
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     753..774
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     RBS             811..833
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 acacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg
       61 ggttgaaggc tctcaagggc atcggtcgac acagaaaagc ccgcctttcg gcgggctttg
      121 ctcgagttag atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
      181 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
      241 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
      301 ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat
      361 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
      421 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
      481 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
      541 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
      601 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
      661 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
      721 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      781 aacaacgcca gtgaaaagct ccatggtata tctccttctt aaagttaaac aaaattattg
      841 ctagctgcaa ccattatcac cgccagaggt aaaattgtca acacgcacgg tgttagctca
      901 gcatgcccag cggaacagca cacac
//
