LOCUS       sequence-149168-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid for building 5n1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Niederholtmeyer H, Sun ZZ, Hori Y, Yeung E, Verpoorte A, Murray RM, 
            Maerkl SJ
  TITLE     Rapid cell-free forward engineering of novel genetic ring 
            oscillators.
  JOURNAL   Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
  PUBMED    26430766
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-10-2"; volume: "4. doi"; pages: " 10.7554/eLife.09771"
COMMENT     Sequence Label: pLacO1-BCD7-tetR-ssrA P42U18C45T13 v40-5a (497)
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    88..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(282..301)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 acgatatccg cctgatgcgt gaacgtgacg gacgtaacga agacaaaacc gcattgcttt
       61 cataaatgtg agcggataac attgacattg tgagcggata acaagatact gagcacagca
      121 agcgggccca agttcactta aaaaggagat caacaatgaa agcaattttc gtactgaaac
      181 atcttaatca tgctggggag ggtttctaat gtctagatta gataaaagta aagtgattaa
      241 cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc
      301 ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt
      361 gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt gccctttaga
      421 aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact
      481 aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga
      541 aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt
      601 atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca
      661 tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg
      721 acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga
      781 attgatcata tgcggattag aaaaacaact taaatgtgaa agtg
//
