LOCUS       sequence-149169-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid for building 5n1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Niederholtmeyer H, Sun ZZ, Hori Y, Yeung E, Verpoorte A, Murray RM, 
            Maerkl SJ
  TITLE     Rapid cell-free forward engineering of novel genetic ring 
            oscillators.
  JOURNAL   Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
  PUBMED    26430766
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-10-2"; volume: "4. doi"; pages: " 10.7554/eLife.09771"
COMMENT     Sequence Label: pLacO1-BCD7-tetR-ssrA P42U18C45T13 v40-5a (497)
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(205..825)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     733..752
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagcga agacaaaagc
       61 cgactctcaa ggttgagaag agaaaagaaa accgccgatc ctgtccaccg cattactgca
      121 aggtagtgga caagaccggc ggtcttaagt tttttggctg aaagttcatt aagctgctaa
      181 agcgtagttt tcgtcgtttg ctgcagaccc actttcacat ttaagttgtt tttctaatcc
      241 gcatatgatc aattcaaggc cgaataagaa ggctggctct gcaccttggt gatcaaataa
      301 ttcgatagct tgtcgtaata atggcggcat actatcagta gtaggtgttt ccctttcttc
      361 tttagcgact tgatgctctt gatcttccaa tacgcaacct aaagtaaaat gccccacagc
      421 gctgagtgca tataatgcat tctctagtga aaaaccttgt tggcataaaa aggctaattg
      481 attttcgaga gtttcatact gtttttctgt aggccgtgta cctaaatgta cttttgctcc
      541 atcgcgatga cttagtaaag cacatctaaa acttttagcg ttattacgta aaaaatcttg
      601 ccagctttcc ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca tctcaatggc
      661 taaggcgtcg agcaaagccc gcttattttt tacatgccaa tacaatgtag gctgctctac
      721 acctagcttc tgggcgagtt tacgggttgt taaaccttcg attccgacct cattaagcag
      781 ctctaatgcg ctgttaatca ctttactttt atctaatcta gacattagaa accctc
//
