LOCUS       sequence-149177-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress p21 in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Lee MS, Seo J, Choi DY, Lee EW, Ko A, Ha NC, Yoon JB, Lee HW, Kim 
            KP, Song J
  TITLE     Stabilization of p21 (Cip1/WAF1) following Tip60-dependent 
            acetylation is required for p21-mediated DNA damage response.
  JOURNAL   Cell Death Differ. 2013 Apr;20(4):620-9. doi: 10.1038/cdd.2012.159. 
            Epub 2012 Dec 14.
  PUBMED    23238566
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cdd.2012.159"; journalName: "Cell Death Differ"; date: 
            "2013-04"; volume: "20"; issue: "4"; pages: "620-9"
COMMENT     Sequence Label: pCS3-MT-BX-6MYC p21
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..551)
                     /codon_start=1
                     /gene="Homo sapiens CDKN1A"
                     /product="human cyclin-dependent kinase inhibitor 1A"
                     /label=p21
                     /note="mediates p53-dependent arrest in the G1 phase of the
                     cell cycle"
                     /translation="MSEPAGDVRQNPCGSKACRRLFGPVDSEQLRRDCDALMAGCIQEA
                     RERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQG
                     TAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRK
                     P"
     CDS             complement(585..614)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(648..677)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(687..716)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(726..755)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(765..794)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atacgactca ctatagttct agatccggtg gatcccgggc ccgcggtacc gtcgagttag
       61 ggcttcctct tggagaagat cagccggcgt ttggagtggt agaaatctgt catgctggtc
      121 tgccgccgtt ttcgaccctg agagtctcca ggtccacctg gggacccttc agcctgctcc
      181 cctgagcgag gcacaagggt acaagacagt gacaggtcca catggtcttc ctctgctgtc
      241 ccctgcagca gagcaggtga ggtgccaggc cgcctgcctc ctcccaactc atcccggcct
      301 cgccggggcc ccgtgggaag gtagagcttg ggcaggccaa ggccccgcac acgctcccag
      361 gcgaagtcac cctccagtgg tgtctcggtg acaaagtcga agttccatcg ctcacgggcc
      421 tcctggatgc agcccgccat tagcgcatca cagtcgcgtc tcagctgctc gctgtccact
      481 gggccgaaga ggcggcggca ggccttgctg ccgcatgggt tctgacggac atccccagcc
      541 ggttctgaca tgaattcgaa gcttgagctc gagatctcta aattcaagtc ctcttcagaa
      601 atgagctttt gctccatggt gaggtcgccc aagctctcca tttcattcaa gtcctcttca
      661 gaaatgagct tttgctccat ttcattcaag tcctcttcag aaatgagctt ttgctccatt
      721 tcattcaagt cctcttcaga aatgagcttt tgctccattt cattcaagtc ctcttcagaa
      781 atgagctttt gctccatttc attcaagtcc tcttca
//
