LOCUS       sequence-149184-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress p21 1-89 in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Lee MS, Seo J, Choi DY, Lee EW, Ko A, Ha NC, Yoon JB, Lee HW, Kim 
            KP, Song J
  TITLE     Stabilization of p21 (Cip1/WAF1) following Tip60-dependent 
            acetylation is required for p21-mediated DNA damage response.
  JOURNAL   Cell Death Differ. 2013 Apr;20(4):620-9. doi: 10.1038/cdd.2012.159. 
            Epub 2012 Dec 14.
  PUBMED    23238566
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cdd.2012.159"; journalName: "Cell Death Differ"; date: 
            "2013-04"; volume: "20"; issue: "4"; pages: "620-9"
COMMENT     Sequence Label: pCS3-MT-BX-6MYC p21 1-89
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        50..68
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     50..67
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             118..147
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             157..186
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             196..225
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             235..264
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             274..303
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             337..366
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    732..866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(756..775)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     810..829
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gctcgtttag ggaaccgcca ttctgcctgg ggacgtcgga gcaagcttga tttaggtgac
       61 actatagaat acaagctact tgttcttttt gcaggatccc atcgatttaa agctatggag
      121 caaaagctca tttctgaaga ggacttgaat gaaatggagc aaaagctcat ttctgaagag
      181 gacttgaatg aaatggagca aaagctcatt tctgaagagg acttgaatga aatggagcaa
      241 aagctcattt ctgaagagga cttgaatgaa atggagcaaa agctcatttc tgaagaggac
      301 ttgaatgaaa tggagagctt gggcgacctc accatggagc aaaagctcat ttctgaagag
      361 gacttgaatt tagagatctc gagctcaagc ttcgaattca tgtcagaacc ggctggggat
      421 gtccgtcaga acccatgcgg cagcaaggcc tgccgccgcc tcttcggccc agtggacagc
      481 gagcagctga gacgcgactg tgatgcgcta atggcgggct gcatccagga ggcccgtgag
      541 cgatggaact tcgactttgt caccgagaca ccactggagg gtgacttcgc ctgggagcgt
      601 gtgcggggcc ttggcctgcc caagctctac cttcccacgg ggccccggcg aggccgggat
      661 gagttgattc tcgacggtac cgcgggcccg ggatccaccg gatctagaac tatagtgagt
      721 cgtattacgt agatccagac atgataagat acattgatga gtttggacaa accacaacta
      781 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      841 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttc
//
