LOCUS       sequence-149185-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress p21 1-89 in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Lee MS, Seo J, Choi DY, Lee EW, Ko A, Ha NC, Yoon JB, Lee HW, Kim 
            KP, Song J
  TITLE     Stabilization of p21 (Cip1/WAF1) following Tip60-dependent 
            acetylation is required for p21-mediated DNA damage response.
  JOURNAL   Cell Death Differ. 2013 Apr;20(4):620-9. doi: 10.1038/cdd.2012.159. 
            Epub 2012 Dec 14.
  PUBMED    23238566
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cdd.2012.159"; journalName: "Cell Death Differ"; date: 
            "2013-04"; volume: "20"; issue: "4"; pages: "620-9"
COMMENT     Sequence Label: pCS3-MT-BX-6MYC p21 1-89
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(360..389)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(423..452)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(462..491)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(501..530)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(540..569)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(579..608)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(658..676)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(659..676)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atacgactca ctatagttct agatccggtg gatcccgggc ccgcggtacc gtcgagaatc
       61 aactcatccc ggcctcgccg gggccccgtg ggaaggtaga gcttgggcag gccaaggccc
      121 cgcacacgct cccaggcgaa gtcaccctcc agtggtgtct cggtgacaaa gtcgaagttc
      181 catcgctcac gggcctcctg gatgcagccc gccattagcg catcacagtc gcgtctcagc
      241 tgctcgctgt ccactgggcc gaagaggcgg cggcaggcct tgctgccgca tgggttctga
      301 cggacatccc cagccggttc tgacatgaat tcgaagcttg agctcgagat ctctaaattc
      361 aagtcctctt cagaaatgag cttttgctcc atggtgaggt cgcccaagct ctccatttca
      421 ttcaagtcct cttcagaaat gagcttttgc tccatttcat tcaagtcctc ttcagaaatg
      481 agcttttgct ccatttcatt caagtcctct tcagaaatga gcttttgctc catttcattc
      541 aagtcctctt cagaaatgag cttttgctcc atttcattca agtcctcttc agaaatgagc
      601 ttttgctcca tagctttaaa tcgatgggat cctgcaaaaa gaacaagtag cttgtattct
      661 atagtgtcac ctaaatcaag cttgctccga cgtccccagg cagaatggcg gttccctaaa
      721 cgagcattgc ttatatagac ctcccattag gcacgcctac cgcccattta cgtcaatgga
      781 acgcccattt gcgtcattgc ccctccccat tgacgtcaat ggggatgtac ttggcagcca
      841 tcgcggg
//
