LOCUS       sequence-149186-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter panel.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Sun ZZ, Yeung E, Hayes CA, Noireaux V, Murray RM
  TITLE     Linear DNA for rapid prototyping of synthetic biological circuits in
            an Escherichia coli based TX-TL cell-free system.
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. 
            Epub 2013 Dec 4.
  PUBMED    24303785
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400131a";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "387-97"
COMMENT     Sequence Label: pBEST-p15A-Pl-tetO1-deGFP-T500 (122p)
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..92
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    99..117
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             141..163
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(200..221)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(461..480)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     808..829
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgatatccgc ctgatgcgtg aacgtgacgg acgtaaccac cgcgacatgt gtgtgctgtt
       61 ccgctgggca tgctccctat cagtgataga gattgacatc cctatcagtg atagagatac
      121 tgagcacagc tagcaataat tttgtttaac tttaagaagg agatatacca tggagctttt
      181 cactggcgtt gttcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      241 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      301 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
      361 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      421 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      481 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      541 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      601 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
      661 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      721 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
      781 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccg
//
