LOCUS       sequence-149190-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GATEWAY destination vector for site-specific integration into the 
            human AAVS1/PPP1R12C locus for doxycycline-inducible gene 
            over-expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Zhu Z, Li QV, Lee K, Rosen BP, Gonzalez F, Soh CL, Huangfu D
  TITLE     Genome Editing of Lineage Determinants in Human Pluripotent Stem 
            Cells Reveals Mechanisms of Pancreatic Development and Diabetes.
  JOURNAL   Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015.
  PUBMED    27133796
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015."
COMMENT     Sequence Label: Puro-iDEST
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(73..90)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    79..303
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    310..391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     335..354
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    575..699
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(842..861)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg atctagaggg cccgtttaaa
       61 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
      121 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
      181 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
      241 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
      301 tgggtcgagg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
      361 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
      421 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
      481 tatggctgat tatgatcctc tagagatatc gtcgacaagc ttatcgatgc ggccgcgcta
      541 gcacgcgtca gctgactaga ggatccccgg atcaaccact ttgtacaaga aagctgaacg
      601 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
      661 tacataatac tgtaaaacac aacatatcca gtcactatgg tcgacctgca gactggctgt
      721 gtataaggga gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt
      781 cattttcgcg gtggctgaga tcagccactt cttccccgat aacggagacc ggcacactgg
      841 ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt
      901 cacgggagac tttatctgac agcagacgtg ca
//
