LOCUS       sequence-149192-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Generates truncated version of MAK10 gene, which is required for 
            dsRNA L-A propagation in S.cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  TITLE     McCusker_pUG-amdSYM-mak10TR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUG-amdSYM-mak10TR
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(81..114)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(165..183)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(166..183)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 aggagggtat tctgggcctc catgtcgctg gccgggtgac ccggcgggga cgaggcaagc
       61 taaacagatc tctagaccta ataacttcgt atagcataca ttatacgaag ttatattaag
      121 ggttgtcgac ctgcagcgta cgaagcttca gctggcggcc gcgttctata gtgtcaccta
      181 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat
      241 atgtccatat gggatgagct aattgaaaac gctaactcgg ttaatggcat atttccacaa
      301 ttacagctgt ctccaccaaa gggtgcgttc tcaacatata tacaaaagca caggtctaat
      361 caattccctc ccaggaagat tacgaagtta ccgacggatt acagcggctt tattacactg
      421 gcaaatgatg ttaaaacaat actccttgta gataaagccg agtctgcgtt ggagacctac
      481 cagtttgcca aattcttcaa caagttggaa caaaggcatg tcattgcaag gatcttattc
      541 cccttatttt tcattagaga cgacagaaca gttttgggta aattttcata tacccaattt
      601 tatttgctac atgtcaagga gttttcagca caaactccat ctgaatttga atcctctatt
      661 ggtaatgaac ttatccaaga aagctctaat atgcttttag aatggtacca aaactgctcc
      721 caaaacacat gtagatatag acaaggtttt aacaggcaac ttattctatg ggattctctt
      781 caagcccagt ttgaaagtgt caattcacaa gtttactgtt cgtggacata ttttatgaaa
      841 ctatcaagta tgatcgaatt ctctttaaag ggg
//
