LOCUS       sequence-149195-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress p21 90-139 in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Lee MS, Seo J, Choi DY, Lee EW, Ko A, Ha NC, Yoon JB, Lee HW, Kim 
            KP, Song J
  TITLE     Stabilization of p21 (Cip1/WAF1) following Tip60-dependent 
            acetylation is required for p21-mediated DNA damage response.
  JOURNAL   Cell Death Differ. 2013 Apr;20(4):620-9. doi: 10.1038/cdd.2012.159. 
            Epub 2012 Dec 14.
  PUBMED    23238566
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cdd.2012.159"; journalName: "Cell Death Differ"; date: 
            "2013-04"; volume: "20"; issue: "4"; pages: "620-9"
COMMENT     Sequence Label: pCS3-MT-BX-6MYC p21 90-139
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        52..70
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     52..69
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             120..149
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             159..188
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             198..227
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             237..266
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             276..305
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             339..368
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    623..757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(647..666)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     701..720
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(870..890)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(870..888)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(909..925)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(909..925)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(922..944)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    933..949
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atgctcgttt agggaaccgc cattctgcct ggggacgtcg gagcaagctt gatttaggtg
       61 acactataga atacaagcta cttgttcttt ttgcaggatc ccatcgattt aaagctatgg
      121 agcaaaagct catttctgaa gaggacttga atgaaatgga gcaaaagctc atttctgaag
      181 aggacttgaa tgaaatggag caaaagctca tttctgaaga ggacttgaat gaaatggagc
      241 aaaagctcat ttctgaagag gacttgaatg aaatggagca aaagctcatt tctgaagagg
      301 acttgaatga aatggagagc ttgggcgacc tcaccatgga gcaaaagctc atttctgaag
      361 aggacttgaa tttagagatc tcgagctcaa gcttcgaatt catgttggga ggaggcaggc
      421 ggcctggcac ctcacctgct ctgctgcagg ggacagcaga ggaagaccat gtggacctgt
      481 cactgtcttg tacccttgtg cctcgctcag gggagcaggc tgaagggtcc ccaggtggac
      541 ctggagactc tcagggtatt ctcgacggta ccgcgggccc gggatccacc ggatctagaa
      601 ctatagtgag tcgtattacg tagatccaga catgataaga tacattgatg agtttggaca
      661 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
      721 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
      781 tatgtttcag gttcaggggg aggtgtggga ggttttttaa ttcgcggccg cggcgccaat
      841 gcattgggcc cggtacccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
      901 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      961 atacgagccg g
//
