LOCUS       sequence-149196-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress p21 90-139 in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Lee MS, Seo J, Choi DY, Lee EW, Ko A, Ha NC, Yoon JB, Lee HW, Kim 
            KP, Song J
  TITLE     Stabilization of p21 (Cip1/WAF1) following Tip60-dependent 
            acetylation is required for p21-mediated DNA damage response.
  JOURNAL   Cell Death Differ. 2013 Apr;20(4):620-9. doi: 10.1038/cdd.2012.159. 
            Epub 2012 Dec 14.
  PUBMED    23238566
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cdd.2012.159"; journalName: "Cell Death Differ"; date: 
            "2013-04"; volume: "20"; issue: "4"; pages: "620-9"
COMMENT     Sequence Label: pCS3-MT-BX-6MYC p21 90-139
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(249..278)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(312..341)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(351..380)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(390..419)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(429..458)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(468..497)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(547..565)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(548..565)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atacgactca ctatagttct agatccggtg gatcccgggc ccgcggtacc gtcgagaata
       61 ccctgagagt ctccaggtcc acctggggac ccttcagcct gctcccctga gcgaggcaca
      121 agggtacaag acagtgacag gtccacatgg tcttcctctg ctgtcccctg cagcagagca
      181 ggtgaggtgc caggccgcct gcctcctccc aacatgaatt cgaagcttga gctcgagatc
      241 tctaaattca agtcctcttc agaaatgagc ttttgctcca tggtgaggtc gcccaagctc
      301 tccatttcat tcaagtcctc ttcagaaatg agcttttgct ccatttcatt caagtcctct
      361 tcagaaatga gcttttgctc catttcattc aagtcctctt cagaaatgag cttttgctcc
      421 atttcattca agtcctcttc agaaatgagc ttttgctcca tttcattcaa gtcctcttca
      481 gaaatgagct tttgctccat agctttaaat cgatgggatc ctgcaaaaag aacaagtagc
      541 ttgtattcta tagtgtcacc taaatcaagc ttgctccgac gtccccaggc agaatggcgg
      601 ttccctaaac gagcattgct tatatagacc tcccattagg cacgcctacc gcccatttac
      661 gtcaatggaa cgcccatttg cgtcattgcc cctccccatt gacgtcaatg gggatgtact
      721 tggcagccat cgcgggccat ttaccgccat tgacgtcaat gggagtactg ccaatgtacc
      781 ctggcgtact tccaatagta atgtacttgc caagttacta ttaatagata ttgatgtact
      841 gccaagtggg ccatttaccg tcattgacgt caataggggg cgtgagaacg gatatgaatg
      901 ggcaatgagc catcccattg acgtcaatgg
//
