LOCUS       sequence-149216-         525 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  delete USP19 gene in human cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Lee JG, Takahama S, Zhang G, Tomarev SI, Ye Y
  TITLE     Unconventional secretion of misfolded proteins promotes adaptation 
            to proteasome dysfunction in mammalian cells.
  JOURNAL   Nat Cell Biol. 2016 Jul;18(7):765-76. doi: 10.1038/ncb3372. Epub 
            2016 Jun 13.
  PUBMED    27295555
REFERENCE   2  (bases 1 to 525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3372"; 
            journalName: "Nat Cell Biol"; date: "2016-07"; volume: "18"; issue: 
            "7"; pages: "765-76"
COMMENT     Sequence Label: USP19 sgRNA1
FEATURES             Location/Qualifiers
     source          1..525
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        135..210
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
       61 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accgagagca
      121 aggatggaga tcctgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
      181 caacttgaaa aagtggcacc gagtcggtgc ttttttgttt tagagctaga aatagcaagt
      241 taaaataagg ctagtccgtt tttagcgcgt gcgccaattc tgcagacaaa tggctctaga
      301 ggtacccgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      361 cccattgacg tcaatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      421 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      481 tattgacgtc aatgacggta aatggcccgc ctggcattgt gccca
//
