LOCUS       sequence-149224-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress RIPK3 S199A in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee 
            HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J
  TITLE     CHIP controls necroptosis through ubiquitylation- and 
            lysosome-dependent degradation of RIPK3.
  JOURNAL   Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 
            2016 Feb 22.
  PUBMED    26900751
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3314"; 
            journalName: "Nat Cell Biol"; date: "2016-03"; volume: "18"; issue: 
            "3"; pages: "291-302"
COMMENT     Sequence Label: pcDNA3-HA-RIPK3 S199A
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             101..127
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac catggcctcc tacccttatg atgtgccaga
      121 ttatgcctct cccgaattct gcagatatcc atcacactgg cggccgctcg agatgtcgtg
      181 cgtcaagtta tggcccagcg gtgcccccgc ccccttggtg tccatcgagg aactggagaa
      241 ccaggagctc gtcggcaaag gcgggttcgg cacagtgttc cgggcgcaac ataggaagtg
      301 gggctacgat gtggcggtca agatcgtaaa ctcgaaggcg atatccaggg aggtcaaggc
      361 catggcaagt ctggataacg aattcgtgct gcgcctagaa ggggttatcg agaaggtgaa
      421 ctgggaccaa gatcccaagc cggctctggt gactaaattc atggagaacg gctccttgtc
      481 ggggctgctg cagtcccagt gccctcggcc ctggccgctc ctttgccgcc tgctgaaaga
      541 agtggtgctt gggatgtttt acctgcacga ccagaacccg gtgctcctgc accgggacct
      601 caagccatcc aacgtcctgc tggacccaga gctgcacgtc aagctggcag attttggcct
      661 gtccacattt cagggaggct cacagtcagg gacagggtcc ggggagccag ggggcaccct
      721 gggctacttg gccccagaac tgtttgttaa cgtaaaccgg aaggccgcca cagccagtga
      781 cgtctacagc ttcgggatcc taatgtgggc agtgcttgct ggaagagaag ttgagttgcc
      841 aaccgaacca tcactcgtgt acgaagcagt gtgcaacagg cagaaccggc cttcattggc
      901 tgagctgccc caagccgggc ctgagactcc cggctta
//
