LOCUS       sequence-149226-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) 
            under the control of the CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, 
            Kramer A, Martens A, Edwards JR, Challen GA
  TITLE     Reprogrammable CRISPR/Cas9-based system for inducing site-specific 
            DNA methylation.
  JOURNAL   Biol Open. 2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067.
  PUBMED    27170255
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Open. 
            2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067."
COMMENT     Sequence Label: pCMV-dCas9-D3A
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             109..129
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             139..162
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 acctctacga aggaggtcct ggacgccaca ctgattcatc agtcaattac ggggctctat
       61 gaaacaagaa tcgacctctc tcagctcggt ggagacagca gggctgaccc caagaagaag
      121 aggaaggtgg gagctagcga ctacaaagac gatgacgaca agggaagccg agaggactgg
      181 ccctcccggc tccagatgtt cttcgctaat aaccacgacc aggaatttga ccctccaaag
      241 gtttacccac ctgtcccagc tgagaagagg aagcccatcc gggtgctgtc tctctttgat
      301 ggaatcgcta cagggctcct ggtgctgaag gacttgggca ttcaggtgga ccgctacatt
      361 gcctcggagg tgtgtgagga ctccatcacg gtgggcatgg tgcggcacca ggggaagatc
      421 atgtacgtcg gggacgtccg cagcgtcaca cagaagcata tccaggagtg gggcccattc
      481 gatctggtga ttgggggcag tccctgcaat gacctctcca tcgtcaaccc tgctcgcaag
      541 ggcctctacg agggcactgg ccggctcttc tttgagttct accgcctcct gcatgatgcg
      601 cggcccaagg agggagatga tcgccccttc ttctggctct ttgagaatgt ggtggccatg
      661 ggcgttagtg acaagaggga catctcgcga tttctcgagt ccaaccctgt gatgattgat
      721 gccaaagaag tgtcagctgc acacagggcc cgctacttct ggggtaacct tcccggtatg
      781 aacaggccgt tggcatccac tgtgaatgat aagctggagc tgcaggagtg tctggagcat
      841 ggcaggatag ccaagttcag caaagtgagg accattacta cgaggtc
//
