LOCUS       sequence-149238-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic 
            domain (CD) under the control of the CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, 
            Kramer A, Martens A, Edwards JR, Challen GA
  TITLE     Reprogrammable CRISPR/Cas9-based system for inducing site-specific 
            DNA methylation.
  JOURNAL   Biol Open. 2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067.
  PUBMED    27170255
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Open. 
            2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067."
COMMENT     Sequence Label: pCMV-dCas9-mD3A
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             116..136
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             146..169
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcggtacacc tctacgaagg aggtcctgga cgccacactg attcatcagt caattacggg
       61 gctctatgaa acaagaatcg acctctctca gctcggtgga gacagcaggg ctgaccccaa
      121 gaagaagagg aaggtgggag ctagcgacta caaagacgat gacgacaagg gaagccgaga
      181 ggactggccc tcccggctcc agatgttctt cgctaataac cacgaccagg aatttgaccc
      241 tccaaaggtt tacccacctg tcccagctga gaagaggaag cccatccggg tgctgtctct
      301 ctttgatgga atcgctacag ggctcctggt gctgaaggac ttgggcattc aggtggaccg
      361 ctacattgcc tcggaggtgt gtgaggactc catcacggtg ggcatggtgc ggcaccaggg
      421 gaagatcatg tacgtcgggg acgtccgcag cgtcacacag aagcatatcc aggagtgggg
      481 cccattcgat ctggtgattg ggggcagtcc ctgcaatgac ctctccatcg tcaaccctgc
      541 tcgcaagggc ctctacgagg gcactggccg gctcttcttt gagttctacc gcctcctgca
      601 tgatgcgcgg cccaaggagg gagatgatcg ccccttcttc tggctctttg cgaatgtggt
      661 ggccatgggc gttagtgaca agagggacat ctcgcgattt ctcgagtcca accctgtgat
      721 gattgatgcc aaagaagtgt cagctgcaca cagggcccgc tacttctggg gtaaccttcc
      781 cggtatgaac aggccgttgg catccactgt gaatgataag ctggagctgc aggagtgtct
      841 ggagcatggc aggatagcca agttcagcaa agtgaggacc attactacga ggtcaaactc
      901 cataaagcag ggcaaagacc agcattttcc tgtcttcatg aatgagaaag aggacatctt
      961 atggtgcact gaaatggaaa gggtatttgg tt
//
