LOCUS       sequence-149241-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress RIPK3 K501R in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee 
            HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J
  TITLE     CHIP controls necroptosis through ubiquitylation- and 
            lysosome-dependent degradation of RIPK3.
  JOURNAL   Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 
            2016 Feb 22.
  PUBMED    26900751
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3314"; 
            journalName: "Nat Cell Biol"; date: "2016-03"; volume: "18"; issue: 
            "3"; pages: "291-302"
COMMENT     Sequence Label: pcDNA3-HA-RIPK3 K501R
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..109
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accatggcct cctaccctta tgatgtgcca gattatgcct ctcccgaatt
      121 ctgcagatat ccatcacact ggcggccgct cgagatgtcg tgcgtcaagt tatggcccag
      181 cggtgccccc gcccccttgg tgtccatcga ggaactggag aaccaggagc tcgtcggcaa
      241 aggcgggttc ggcacagtgt tccgggcgca acataggaag tggggctacg atgtggcggt
      301 caagatcgta aactcgaagg cgatatccag ggaggtcaag gccatggcaa gtctggataa
      361 cgaattcgtg ctgcgcctag aaggggttat cgagaaggtg aactgggacc aagatcccaa
      421 gccggctctg gtgactaaat tcatggagaa cggctccttg tcggggctgc tgcagtccca
      481 gtgccctcgg ccctggccgc tcctttgccg cctgctgaaa gaagtggtgc ttgggatgtt
      541 ttacctgcac gaccagaacc cggtgctcct gcaccgggac ctcaagccat ccaacgtcct
      601 gctggaccca gagctgcacg tcaagctggc agattttggc ctgtccacat ttcagggagg
      661 ctcacagtca gggacagggt ccggggagcc agggggcacc ctgggctact tggccccaga
      721 actgtttgtt aacgtaaacc ggaaggcctc cacagccagt gacgtctaca gcttcgggat
      781 cctaatgtgg gcagtgcttg ctggaagaga agttgagttg ccaaccgaac catcactcgt
      841 gtacgaagca gtgtgcaaca ggcagaaccg gccttcattg gctga
//
