LOCUS       sequence-149244-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress RIPK1 K571R in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee 
            HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J
  TITLE     CHIP controls necroptosis through ubiquitylation- and 
            lysosome-dependent degradation of RIPK3.
  JOURNAL   Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 
            2016 Feb 22.
  PUBMED    26900751
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3314"; 
            journalName: "Nat Cell Biol"; date: "2016-03"; volume: "18"; issue: 
            "3"; pages: "291-302"
COMMENT     Sequence Label: pcDNA3-HA-RIPK1 K571R
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             101..127
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac catggcctcc tacccttatg atgtgccaga
      121 ttatgcctct cccgaattca tgcaaccaga catgtccttg aatgtcatta agatgaaatc
      181 cagtgacttc ctggagagtg cagaactgga cagcggaggc tttgggaagg tgtctctgtg
      241 tttccacaga acccagggac tcatgatcat gaaaacagtg tacaaggggc ccaactgcat
      301 tgagcacaac gaggccctct tggaggaggc gaagatgatg aacagactga gacacagccg
      361 ggtggtgaag ctcctgggcg tcatcataga ggaagggaag tactccctgg tgatggagta
      421 catggagaag ggcaacctga tgcacgtgct gaaagccgag atgagtactc cgctttctgt
      481 aaaaggaagg ataattttgg aaatcattga aggaatgtgc tacttacatg gaaaaggcgt
      541 gatacacaag gacctgaagc ctgaaaatat ccttgttgat aatgacttcc acattaagat
      601 cgcagacctc ggccttgcct cctttaagat gtggagcaaa ctgaataatg aagagcacaa
      661 tgagctgagg gaagtggacg gcaccgctaa gaagaatggc ggcaccctct actacatggc
      721 gcccgagcac ctgaatgacg tcaacgcaaa
//
