LOCUS       sequence-149263-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress RIPK3 V460P in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee 
            HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J
  TITLE     CHIP controls necroptosis through ubiquitylation- and 
            lysosome-dependent degradation of RIPK3.
  JOURNAL   Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 
            2016 Feb 22.
  PUBMED    26900751
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3314"; 
            journalName: "Nat Cell Biol"; date: "2016-03"; volume: "18"; issue: 
            "3"; pages: "291-302"
COMMENT     Sequence Label: pcDNA3-HA-RIPK3 V460P
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             100..126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc atggcctcct acccttatga tgtgccagat
      121 tatgcctctc ccgaattctg cagatatcca tcacactggc ggccgctcga gatgtcgtgc
      181 gtcaagttat ggcccagcgg tgcccccgcc cccttggtgt ccatcgagga actggagaac
      241 caggagctcg tcggcaaagg cgggttcggc acagtgttcc gggcgcaaca taggaagtgg
      301 ggctacgatg tggcggtcaa gatcgtaaac tcgaaggcga tatccaggga ggtcaaggcc
      361 atggcaagtc tggataacga attcgtgctg cgcctagaag gggttatcga gaaggtgaac
      421 tgggaccaag atcccaagcc ggctctggtg actaaattca tggagaacgg ctccttgtcg
      481 gggctgctgc agtcccagtg ccctcggccc tggccgctcc tttgccgcct gctgaaagaa
      541 gtggtgcttg ggatgtttta cctgcacgac cagaacccgg tgctcctgca ccgggacctc
      601 aagccatcca acgtcctgct ggacccagag ctgcacgtca agctggcaga ttttggcctg
      661 tccacatttc agggaggctc acagtcaggg acagggtccg gggagccagg gggcaccctg
      721 ggctacttgg ccccagaact gtttgttaac gtaaaccgga aggcctccac agccagtgac
      781 gtctacagct tcgggatcct aatgtgggca gtgcttgctg gaagagaagt tgagttgcca
      841 accgaaccat cactcgtgta cgaagcagtg tgcaacaggc agaaccgg
//
