LOCUS       sequence-149275-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress RIPK3 K50A in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee 
            HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J
  TITLE     CHIP controls necroptosis through ubiquitylation- and 
            lysosome-dependent degradation of RIPK3.
  JOURNAL   Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 
            2016 Feb 22.
  PUBMED    26900751
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3314"; 
            journalName: "Nat Cell Biol"; date: "2016-03"; volume: "18"; issue: 
            "3"; pages: "291-302"
COMMENT     Sequence Label: pcDNA3-FLAG-RIPK3 K50A
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      71..80
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             80..103
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgcc gccaccatgg attacaagga tgacgacgat aagagcgcgg gcggatccga
      121 attctgcaga tatccatcac actggcggcc gctcgagatg tcgtgcgtca agttatggcc
      181 cagcggtgcc cccgccccct tggtgtccat cgaggaactg gagaaccagg agctcgtcgg
      241 caaaggcggg ttcggcacag tgttccgggc gcaacatagg aagtggggct acgatgtggc
      301 ggtcgcgatc gtaaactcga aggcgatatc cagggaggtc aaggccatgg caagtctgga
      361 taacgaattc gtgctgcgcc tagaaggggt tatcgagaag gtgaactggg accaagatcc
      421 caagccggct ctggtgacta aattcatgga gaacggctcc ttgtcggggc tgctgcagtc
      481 ccagtgccct cggccctggc cgctcctttg ccgcctgctg aaagaagtgg tgcttgggat
      541 gttttacctg cacgaccaga acccggtgct cctgcaccgg gacctcaagc catccaacgt
      601 cctgctggac ccagagctgc acgtcaagct ggcagatttt ggcctgtcca catttcaggg
      661 aggctcacag tcagggacag ggtccgggga gccagggggc accctgggct acttggcccc
      721 agaactgttt gttaacgtaa accggaaggc ctccacagcc agtgacgtct acagcttcgg
      781 gatcctaatg tgggcagtgc ttgctggaag agaagttgag ttgccaaccg aaccatcact
      841 cgtgtacgaa gcagtgtgca acaggcagaa ccggccttca ttggctgagc tgccccaagc
      901 cggg
//
