LOCUS       sequence-149281-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress RIPK1 K115R in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee 
            HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J
  TITLE     CHIP controls necroptosis through ubiquitylation- and 
            lysosome-dependent degradation of RIPK3.
  JOURNAL   Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 
            2016 Feb 22.
  PUBMED    26900751
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3314"; 
            journalName: "Nat Cell Biol"; date: "2016-03"; volume: "18"; issue: 
            "3"; pages: "291-302"
COMMENT     Sequence Label: pcDNA3-FLAG-RIPK1 K115R
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             94..117
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tgccgccacc atggattaca aggatgacga cgataagagc
      121 gcgggcggat ccgaattcat gcaaccagac atgtccttga atgtcattaa gatgaaatcc
      181 agtgacttcc tggagagtgc agaactggac agcggaggct ttgggaaggt gtctctgtgt
      241 ttccacagaa cccagggact catgatcatg aaaacagtgt acaaggggcc caactgcatt
      301 gagcacaacg aggccctctt ggaggaggcg aagatgatga acagactgag acacagccgg
      361 gtggtgaagc tcctgggcgt catcatagag gaagggaagt actccctggt gatggagtac
      421 atggagaagg gcaacctgat gcacgtgctg aaagccgaga tgagtactcc gctttctgta
      481 agaggaagga taattttgga aatcattgaa ggaatgtgct acttacatgg aaaaggcgtg
      541 atacacaagg acctgaagcc tgaaaatatc cttgttgata atgacttcca cattaagatc
      601 gcagacctcg gccttgcctc ctttaagatg tggagcaaac tgaataatga agagcacaat
      661 gagctgaggg aagtggacgg caccgctaag aagaatggcg gcaccctcta ctacatggcg
      721 cccgagcacc tgaatgacgt caacgcaaag cccacagaga agtcggatgt gtacagcttt
      781 gctgtagtac tctgggcgat atttgcaaat aaggagccat atgaa
//
