LOCUS       sequence-149293-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress RIPK1 K163R in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee 
            HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J
  TITLE     CHIP controls necroptosis through ubiquitylation- and 
            lysosome-dependent degradation of RIPK3.
  JOURNAL   Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 
            2016 Feb 22.
  PUBMED    26900751
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3314"; 
            journalName: "Nat Cell Biol"; date: "2016-03"; volume: "18"; issue: 
            "3"; pages: "291-302"
COMMENT     Sequence Label: pcDNA3-FLAG-RIPK1 K163R
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc ttgccgccac catggattac aaggatgacg acgataagag
      121 cgcgggcgga tccgaattca tgcaaccaga catgtccttg aatgtcatta agatgaaatc
      181 cagtgacttc ctggagagtg cagaactgga cagcggaggc tttgggaagg tgtctctgtg
      241 tttccacaga acccagggac tcatgatcat gaaaacagtg tacaaggggc ccaactgcat
      301 tgagcacaac gaggccctct tggaggaggc gaagatgatg aacagactga gacacagccg
      361 ggtggtgaag ctcctgggcg tcatcataga ggaagggaag tactccctgg tgatggagta
      421 catggagaag ggcaacctga tgcacgtgct gaaagccgag atgagtactc cgctttctgt
      481 aaaaggaagg ataattttgg aaatcattga aggaatgtgc tacttacatg gaaaaggcgt
      541 gatacacaag gacctgaagc ctgaaaatat ccttgttgat aatgacttcc acattaagat
      601 cgcagacctc ggccttgcct cctttaggat gtggagcaaa ctgaataatg aagagcacaa
      661 tgagctgagg gaagtggacg gcaccgctaa gaagaatggc ggcaccctct actacatggc
      721 gcccgagcac ctgaatgacg tcaacgcaaa gcccacagag aagtcggatg tgtacagctt
      781 tgctgtagta ctctgggcga tatttgcaaa taaggagcca tatgaa
//
