LOCUS       sequence-149300-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress RIPK1 K316R in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee 
            HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J
  TITLE     CHIP controls necroptosis through ubiquitylation- and 
            lysosome-dependent degradation of RIPK3.
  JOURNAL   Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 
            2016 Feb 22.
  PUBMED    26900751
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3314"; 
            journalName: "Nat Cell Biol"; date: "2016-03"; volume: "18"; issue: 
            "3"; pages: "291-302"
COMMENT     Sequence Label: pcDNA3-FLAG-RIPK1 K316R
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             93..116
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt gccgccacca tggattacaa ggatgacgac gataagagcg
      121 cgggcggatc cgaattcatg caaccagaca tgtccttgaa tgtcattaag atgaaatcca
      181 gtgacttcct ggagagtgca gaactggaca gcggaggctt tgggaaggtg tctctgtgtt
      241 tccacagaac ccagggactc atgatcatga aaacagtgta caaggggccc aactgcattg
      301 agcacaacga ggccctcttg gaggaggcga agatgatgaa cagactgaga cacagccggg
      361 tggtgaagct cctgggcgtc atcatagagg aagggaagta ctccctggtg atggagtaca
      421 tggagaaggg caacctgatg cacgtgctga aagccgagat gagtactccg ctttctgtaa
      481 aaggaaggat aattttggaa atcattgaag gaatgtgcta cttacatgga aaaggcgtga
      541 tacacaagga cctgaagcct gaaaatatcc ttgttgataa tgacttccac attaagatcg
      601 cagacctcgg ccttgcctcc tttaagatgt ggagcaaact gaataatgaa gagcacaatg
      661 agctgaggga agtggacggc accgctaaga agaatggcgg caccctctac tacatggcgc
      721 ccgagcacct gaatgacgtc aacgcaaagc ccacagagaa gtcggatgtg tacagctttg
      781 ctgtagtact ctgggcgata tttgcaaata aggagccata tgaa
//
