LOCUS       sequence-149320-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress PPARgamma2 in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Kim JH, Park KW, Lee EW, Jang WS, Seo J, Shin S, Hwang KA, Song J
  TITLE     Suppression of PPARgamma through MKRN1-mediated ubiquitination and 
            degradation prevents adipocyte differentiation.
  JOURNAL   Cell Death Differ. 2014 Apr;21(4):594-603. doi: 
            10.1038/cdd.2013.181. Epub 2013 Dec 13.
  PUBMED    24336050
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cdd.2013.181"; journalName: "Cell Death Differ"; date: 
            "2014-04"; volume: "21"; issue: "4"; pages: "594-603"
COMMENT     Sequence Label: pcDNA3.1-PPARgamma2
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttggta ccgagctcgg
      121 atccactagt ccagtgtggt ggaattctgc agatatccag cacagtggcg gccgctcgag
      181 atgggtgaaa ctctgggaga ttctcctgtt gacccagagc atggtgcctt cgctgatgca
      241 ctgcctatga gcacttcaca agaaattacc atggttgaca cagagatgcc attctggccc
      301 accaacttcg gaatcagctc tgtggacctc tccgtgatgg aagaccactc gcattccttt
      361 gacatcaagc cctttaccac agttgatttc tccagcattt ctgctccaca ctatgaagac
      421 attccattca caagagctga cccaatggtt gctgattaca aatatgacct gaagctccaa
      481 gaataccaaa gtgcgatcaa agtagaacct gcatctccac cttattattc tgaaaagacc
      541 cagctctaca acaggcctca tgaagaacct tctaactccc tcatggccat tgagtgccga
      601 gtctgtgggg ataaagcatc aggcttccac tatggagttc atgcttgtga aggatgcaag
      661 ggttttttcc gaagaaccat ccgattgaag cttatttatg ataggtgtga tcttaactgc
      721 cggatccaca aaaaaagtag aaataaatgt cagtactgtc ggtttcagaa gtgccttgct
      781 gtggggatgt ctcacaatgc catcaggttt gggcggatgc cacaggccga gaaggagaag
      841 ctgttggcgg agatctccag tgatatcgac cagctgaacc cagagtctgc tgatctgcga
      901 gccctggcaa agcatttgta tg
//
